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Cloning and expression of glutathione S-transferase (GST) cDNA from Gossypium hirsutum L. KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/254841
한국약용작물학회지
(Korean Journal of Medical Crop Science)
Vol.10 No.4 (2002.11)
pp.294-297
pp.294-297
한국약용작물학회
(The Korean Society of Medicinal Crop Science)
초록
A gene coding for the GST of cotton (Gh-5) was cloned into Escherichia coli and experssed. The enzyme remained within the cytoplasm of E. coli. An 696 bp open reading frame was in the 988 base pair fragment of the recombinant plasmid pET-30b(+). The deduced protein sequence consists of 232 amino acids and has a molecular mass of 30235.58 Da. The cloned enzyme conjugated reduced glutathione and 1-chloro-2,4-dinitrobenzene (CDNB). Plant GST cDNA was expressed in microbe and produced polypeptide had function as an enzyme.
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