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오미자의 현탁배양세포로부터 Gomisin J의 생산 KCI 등재

Production of Gomisin J from Cell Suspension Cultures of Schisandra chinensis Baillon

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한국약용작물학회지 (Korean Journal of Medical Crop Science)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
초록

Cell growth and gomisin J production by suspension cultures of Schisandra chinensis Baillon were investigated under various culture media, initial sucrose concentrations, shaking speeds, and inoculum sizes. Callus was induced from in vitro cultivated leaf segments on MS medium supplemented with 1 mg/l NAA. The maximum dry cell weight of 2.23 g was obtained at inoculum size of 0.5 g fresh cell weight and in MB5 medium supplemented with 1 mg/l NAA, 3% sucrose after 8 weeks. The production of gomisin J in suspension cell cultures was maximized in WPM medium containing 5% sucrose. The shaking speed for maintaining maximal cell dry weight was 100 rpm while the best shaking speed for gomisin J accumulation was 140 rpm.

저자
  • 황성진 | Hwang, Sung-Jin
  • 표병식 | Pyo, Byoung-Sik
  • 이학주 | Lee, Hak-Ju
  • 황백 | Hwang, Baik