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들깨 Limonene 유전자의 담배식물(Nicotiana benthamiana)내 Agroinfiltration에 의한 분자적 특성 KCI 등재
Molecular Characterization of the Perilla frutescens Limonene Gene (PFLS) by Agroinfiltration into Nicotiana benthamiana
pp.33-38
The full-length cDNA encoding Perilla frutescens limonene synthase (PFLS) (603 amino acids, GenBank accession no. D49368) was cloned. To elucidate the role of PFLS in gene regulation, we transiently transformed full-length PFLS into tobacco plants. PFLS mRNA was first detected in the intact leaves of the plants at 6 h, and the LS transcript level increased after 12 h in leaves treated with oxidative stress-related chemicals. The transient overexpression of PFLS resulted in increased transcription of NbPR1 and NbSIP in Nicotiana benthamiana leaves. Thus, our result confirmed that the infiltration of PFLS gene act as a transcriptional regulator of NbPR1 or NbSIP genes in the tobacco.
