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PNA 바이오칩을 이용한 고려인삼 품종 판별 KCI 등재

Molecular Identification of Korean Ginseng Cultivars (Panax ginseng C. A. Mey.) using Peptide Nucleic Acid (PNA) Microarray

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한국약용작물학회지 (Korean Journal of Medical Crop Science)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
초록

This study was carried out to identify Korean ginseng cultivars using peptide nucleic acid (PNA) microarray. Sixty-seven probes were designed based on nucleotide variation to distinguish Korean ginseng cultivars of Panax ginseng. Among those PNA probes, three (PGB74, PGB110 and PGB130) have been developed to distinguish five Korean ginseng cultivars. Five Korean ginseng cultivars were denoted as barcode numbers depending on their fluorescent signal patterns of each cultivar using three probe sets in the PNA microarray. Five Korean ginseng cultivars, Chunpoong, Yunpoong, Gopoong, Gumpoong and Sunpoong, were simply denoted as '111', '222', '211', '221' and '122', respectively. This is the first report of PNA microarray which provided an objective and reliable method for the authentication of Korean ginseng cultivars. Also, the PNA microarray will be useful for management system and pure guarantee in ginseng seed.

저자
  • 방경환 | Bang, Kyong-Hwan
  • 조익현 | Jo, Ick-Hyun
  • 김영창 | Kim, Young-Chang
  • 김장욱 | Kim, Jang-Uk
  • 박홍우 | Park, Hong-Woo
  • 신미란 | Shin, Mi-Ran
  • 김영배 | Kim, Young-Bae
  • 김옥태 | Kim, Ok-Tae
  • 현동윤 | Hyun, Dong-Yun
  • 김동휘 | Kim, Dong-Hwi
  • 차선우 | Cha, Seon-Woo