Effect of initial PH on the lipid accumulation and fatty acid composition of some species of mold were investigated. The maximum lipid content content produced by Aspergillus niger var. macrosporus was 17.5% at PH 3.5, by Aspergillus fumigatus 23.5% at PH 3.5 by Penicillium spinulosum 12.0% at PH 4.0 and by Penicillium notatum 7.3% at PH 4.0. The major fatty acids were palmitic, stearic, oleic and linoleic acid in all experimental molds. At PH from 4.5 to 6.0, the proportion of linoleic acid was increased and those of palmitic and oleic acid were decreased with rising in PH, therefore, degree of lipid unsaturation was increased in all experimental molds.