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입본특이적(立本特異的) 방법(方法)과 13C-NMR 기법(技法)에 의한 잣기름의 트리아실 글리세롤의 구성지방산(構成脂肪酸)의 분포(分布)에 관한 연구(硏究) KCI 등재
Studies on the Fatty Acid Distribution in the Position of Triacylglycerols from the Seed of Pinus Koraiensis by Stereo-specific Analysis and 13C-NMR Techniques
pp.35-44
All the triacylglycerols including the molecular species having δ5-unsaturated fatty acids from the seeds of Pinus Koraiensis, were split into a mixture of diacylglycerols by a Grignard reagent prepared with allyl bromide without arousing acyl chains of a glycerol moiety to migration, and were also easily partially hydrolyzed to diacylglycerols by pancreatic lipase. (S)-(+)-(1-naphthyl)ethyl urethane(NEU) derivatives of the diacylglycerol mixture derived from the triacylglycerols were fractionated into sn-1, 3-, sn-1, 2- and sn-2, 3-DG-NEU by silica-HPLC and the fatty acid composition of these fractions was analysed. C18:1Ω9 is distributed evenly in the three positions of TG with C18:2Ω6 mainly located in sn-2 position, while δ5-unsaturated fatty acids such as δ5.9-C18:2, δ5.9.12-C18:3 and δ5.11.14-C20:3 are exclusively present in the sn-3 position. These results could be confirmed by 13C-NMR spectroscopy : the signals at δ173.231 ppm and δ172.811 ppm of the carbonyl carbon of acyl moieties indicate the presence of saturated acids and/or C18:1Ω9 (oleic acid) in the α(α')- or β- positions, and C18:2Ω6 including C18:1Ω9 in the β-position, respectively. In addition, the resonance at δ173.044 ppm suggested a location of δ5-unsaturated fatty acid moiety in the α(α')-position.
