인삼 부정근의 생장과 ginsenosides 함량에 미치는 elicitor의 영향을 조사한 결과 chitosan처리구의 생장량은 10 mg/L에서 가장 양호하였으며, ginsenosides의 함량은 5 mg/L처리농도에서 14.48 mg/g . DW로 대조구에 비해 약9%의 함량증대를 가져왔다. 세포내에서 이차대사계를 활성화시키는 중간 신호전달 물질(signal transducer)로 알려진 jasmonic acid를 인삼 부정근 배양에 처리한 결과 부정근의 생장량은 10 uM 처리구에서 ginsenosides함량이 가장 양호하였으나, 인삼 부정근의 생장은 다소 억제되었다.

In order to investigate the effects of elicitors on the growth and ginsenosides biosynthesis of ginseng adventitious roots, chitosan and jasmonic acid were treated with various concentrations. The growth rate of adventitious roots was increased with the addition of chitosan at higher concentrations (10 mg/L), but the best accumulation of ginsenosides was observed at the lower concentration (5 mg/L). Jasmonic acid was an effective elicitor for ginsenosides biosynthesis in ginseng adventitious roots. The maximum accumulation of ginsenosides was observed at the treatment of 10 uM jasmonic acid. But the jasmonic acid was found to decrease the growth rate of adventitious roots.

• 이범수
• 인준교
• 송원섭
• 양덕춘