Eco-frendly Control of Culex pipiens (mosquito) Larvae by Acorus calamus (sweet flag) and Acorus gramineus (Grassy-leaved sweet flag) Extracts
Mosquitoes are carriers of malaria and encephalitis. This study performed for eco-friendly control of mosquitos by using genus Acorus. Several solvents were used for the extraction of genus Acorus; water, ethanol, and methanol. Grinded leaves and roots were also included. Acorus extracts killed mosquito larvae and the ethanol extract showed the best result. Autoclaved Acorus water needed long time to kill mosquito larvae. LT50 of 1 % Acorus calamus decoction was 13.6 hrs and 1 % autoclaved Acorus water was 53.6 hrs. LT50 of 0.05% Acorus calamus rhizome powder was 28.5 hrs. LT50 of 0.5% Acorus calamus leaf powder was 10.8 hrs. LT50 of 0.1 % Acorus calamus decoction was 63.4 hrs and 0.1 % Acorus calamus ethanol extracts was 48.6 hrs and 0.1% Acorus calamus methanol extracts was 53.9 hrs. LT50 of 0.4% Acorus gramineus decoction was 45.5 hrs, 0.4% ethanol extracts was 10.9 hrs, 0.4% methanol extracts was 10.2 hrs. LT50 of ethanol extracts was shorter than other extracts. Acorus calamus rhizome powder could be used for the eco-friendly control of the mosquito larvae.