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대량의 쌀 시료 분석을 위한 DNA 추출법 KCI 등재
High-Throughput DNA Extraction Method for Marker Analysis in Rice Grain
- 언어KOR
- URLhttps://db.koreascholar.com/Article/Detail/263604
pp.269-273
The study of molecular markers to improve crops largely depends on the availability of rapid and of efficient DNA extraction methods. Here we developed a cheap and convenient method to isolate genomic DNA from rice grains suitable for large-scale microsatellite analysis. We confirmed that the isolated rice DNA is suitable for PCR analysis with STS marker and SNP marker, as well as microsatellite marker. Further, we established high-throughput DNA extraction system in a 96-well plate format which make it possible high-throughput analysis of microsatellite markers with rice grains. This implies that the new method could be a useful tool for other types of marker analysis in large scale.
