The purpose of this sudy is to get characteristics of water quality in separated sewer overflows. In dry weather the water quality of sewage in the outfall of the separated sewer was an average BOD of 2.7 mg/L, CODmn of 4.9 mg/L, TN of 3.8 mg/L, TP of 1.9 mg/L, and SS of 5.3 mg/L, Because the quality except for TP was less than the water quality standard of wastewater treatment plant (WTP), a prevention facility was needed to prevent the sewage inflow to interceptor. When the separated sewer overflows (SSO) were overflowed, the maximum pollutant load was be found to be about from 2.7 to 5.4 times as a BOD base, about from 3.2 to 4.6 times as a SS base higher than average load. There was a big difference according to rainfall intensity and rainfall continuous time. In case the quality of SSO was less than water quality standard or water quality in dry season, SSO was required to discharge to the stream not to inflow to WTP by operating the floodgate in the outfall with water quality data using sensors, such as turbidity sensor, etc. Because removal efficiency by an intercepter load was calculated as low as about 10~20% as a BOD base, removal efficiency could be increased by installing a separated wall within the sewer.