Biogas is a clean environment friendly fuel that is produced by bacterial conversion of organic matter underanaerobic condition. Raw biogas contains about 55~65% methane, 30~45% carbon dioxide, 0.5% of hydrogensulfide gas and fraction of water vapor. Pure methane has a caloric value of 9,800 kcal/m3, but the caloric value ofraw biogas is about 5,000kcal/m3. To achieve the standard composition of the biogas the treatment techniques likeabsorption or membrane separation must be applied. In this paper the experimental results of the methane purificationin simulated biogas mixture consisted of methane, carbon dioxide and hydrogen sulfide were presented. It was shownthat the using the hollow fiber module with polysulfone membrane it was possible to achieve the purification ofmethane from the concentration of 55% up to 97%. The membrane material was resistant to the small concentrationsof sour gases and for the prevention of methane losses the membrane system has to be used to improve processefficiency.