To determine the optimal media conditions for the detection of the cellulolytic activity in Ganoderma lucidum, we varied three media conditions: dye reagent, pH, and temperature. First, we evaluated the use of four dyes, Congo Red, Phenol Red, Remazol Brilliant Blue, and Trypan Blue. To observe the effect of pH on the chromogenic reaction, we also made and tested various media spanning acidic and alkaline pHs, ranging from 4.5 to 8.0. Furthermore, in order to research the effect of temperature on the clear zone and the fungus growing zone, we tested temperatures ranging from 15 to 35℃. On the whole, the best protocol called for Ganoderma lucidum transfer onto media containing Congo red with pH adjusted to 7.0, followed by incubation at 25℃ for 5 days. Our results will be useful to researchers who aim to study extracellular enzyme activity in Ganoderma lucidum

• Woo-Sik Jo(Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration, Daegu 702-320, Korea)
• Soon-Hwa Bae( Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration, Daegu 702-320, Korea) | Soon-Hwa Bae
• Doo-Hyun Cho( Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration, Daegu 702-320, Korea) | Doo-Hyun Cho
• So-Deuk Park( Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration, Daegu 702-320, Korea) | So-Deuk Park
• Young-Bok Yoo( Mushroom Research Division Department of Herbal Crop Research National Inst. of Horicultural & Herbal Science, RDA, Suwon 404-707, Korea) | Young-Bok Yoo
• Seung-Chun Park( College of Veterinary Medicine, Kyeongbuk National University, Daegu 702-701, Korea) | Seung-Chun Park