A new commercial strain "Dan Bi" of Pleurotus eryngii was developed by intra-specific crossing between monokaryotic spores derived from KNR2312 and KNR2596, respectively. The optimum temperature of mycelial growth was 25℃ and that of fruiting body development was 15 -16℃. The primordia formation of "Dan Bi" was one day later than that of control strain KNR2312. The color of pileus and stipe surface was neutral-brown and pure white, respectively. The shape of pileus was dome and has a scale like as cobweb. The yield was 93±4.8g/850cc plastic bottle. Analysis of the genetic characteristics of the new commercial strain "Dan Bi" showed a different profile as that of the control strain KNR2312 when RAPD primer 8001, 8002, 8003, 8004, and 8005 were used. This new variety of King Oyster mushroom is characterized by a few primordia formation after scratching. It would be of much help to mushroom cultivators in a process of culling of P. eryngii.

• Min-Keun Kim(Environment-friendly Research Division, Gyeongnam Agricultural Research and Extension Services, Jinju 660-360, Korea)
• Jae-San Ryu( Environment-friendly Research Division, Gyeongnam Agricultural Research and Extension Services, Jinju 660-360, Korea) | Jae-San Ryu
• Ki-Kwan Park( Environment-friendly Research Division, Gyeongnam Agricultural Research and Extension Services, Jinju 660-360, Korea) | Ki-Kwan Park
• Hee-Jeong Je( Environment-friendly Research Division, Gyeongnam Agricultural Research and Extension Services, Jinju 660-360, Korea) | Hee-Jeong Je
• Seon-Gi Jeong( Environment-friendly Research Division, Gyeongnam Agricultural Research and Extension Services, Jinju 660-360, Korea) | Seon-Gi Jeong
• Won-Doo Song( Environment-friendly Research Division, Gyeongnam Agricultural Research and Extension Services, Jinju 660-360, Korea) | Won-Doo Song
• Chi-Woong Rho( Environment-friendly Research Division, Gyeongnam Agricultural Research and Extension Services, Jinju 660-360, Korea) | Chi-Woong Rho
• Young-Bok Yoo( Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) | Young-Bok Yoo