Spent mushroom substrate(SMS) is a by-product remaining after a crop of mushrooms. About 9 thermophilic strains were isolated from SMS(Flammulina velvtipes). Among of them, one isolate, designated UJ09, showed the antifungal activity against Aspergillus flavus and Aspergillus ochraceous producing mycotoxin on PDA medium, potentially. The strain UJ09 was produced cellulase and xylanase as hydrolase. The strain UJ21 was identified as members of the genus Bacillus by biochemical characteristics using Bacillus ID kit and VITEK 2 system. Comparative 16S rDNA gene sequence analysis showed that strain UJ09 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to Bacillus amyloliquefaciens with 16S rDNA gene sequence similarity of 99.2%. On the basis of its physiological properties, biochemical characteristics and phylogenetic distinctiveness, strain UJ09 was classified within the genus Bacillus, for which the name Bacillus amyloliquefaciens UJ09 is proposed.

• Yun Jin Cho(Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea)
• Pyung Gyun Shin( Division of Mushroom Research, International Institute of Horticultural & Herhal Science, RDA, Suwon, 441-707, Korea) | Pyung Gyun Shin
• Young Bok Yoo( Division of Mushroom Research, International Institute of Horticultural & Herhal Science, RDA, Suwon, 441-707, Korea) | Young Bok Yoo
• Young Un Cho( Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea) | Young Un Cho
• Soo Jeong Cho( Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea) | Soo Jeong Cho