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Morphological Features of the Mycelia and Fruit Body of Beauveria bassiana Grown on Different Media and Cultivated on Artificial Cultivation

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  • URLhttps://db.koreascholar.com/Article/Detail/277032
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한국버섯학회 (The Korean Society of Mushroom Science)
초록

Beauveria bassiana is one of universal insect pathogenic fungi that have been used for biocontrol agent against insect pests. This fungus has also been studied for medicinal use. To meet for commercial use, the artificial production of the fruit body of this fungus has been established by the Mushtech Co in Korea. This study was carried out to define the morphological features of the fruit body of B. bassiana developed through artificial cultivation. For the observation of mycelia growth, B. bassiana was cultured on the Sabouraud Dextrose agar plus Yeast Extract(SDAY), nut-supplemented medium, and Fe ion-supplemented SDAY at 25℃ for 15 days. The variation of colony color was observed between the different media. Strong pigmentation was observed on Fe ion-supplemented SDAY. To investigate morphological characteristics of fruit body, geminating ascospores and vegetative hyphae were observed though light microscopy and scanning microscope. During seven weeks of cultivation period, the development process of apical fertile part of stromata can be separated by the development stage of perithecia. To understand the developing process of fruit body at the transcript level, investigating process of distinct gene expression according to cultural condition and developmental stage was discussed.

저자
  • Seong Hwan Kim( Department of Microbiology, Dankook University) | Seong Hwan Kim
  • Gi-Ho Sung( Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) | Gi-Ho Sung
  • Junsang Oh( Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) | Junsang Oh
  • Jun Yong Kim( Department of Microbiology, Dankook University) | Jun Yong Kim
  • Min Woo Hyun( Department of Microbiology, Dankook University) | Min Woo Hyun
  • Hyuk Woo KWON(Department of Microbiology, Dankook University)