Spent mushroom substrate (SMS) is a by-product remaining after a crop of mushrooms. The fresh SMS was sample within 24 hours of removal from the production facility in DOJUN farm located in Jinju. About 11 bacterial species were isolated from fresh SMS on TSA medium. Among of them, one isolate, designated YJ02, showed the antifungal activity against Aspergillus flavus and Aspergillus ochraceous producing mycotoxin on PDA medium, potentially. The strain YJ02 was produced cellulase, xylanase, mannanase as hydrolase. The strain YJ02 was identified as members of the genus Bacillus by biochemical characteristics using Bacillus ID kit and VITEK 2 system. Comparative 16S rDNA gene sequence analysis showed that strain YJ02 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to Bacillus amyloliquefaciens with 16S rDNA gene sequence similarity of 98.7%. On the basis of its physiological properties, biochemical characteristics and phylogenetic distinctiveness, strain YJ02 was classified within the genus Bacillus, for which the name Bacillus amyloliquefaciens YJ02 is proposed.

• Yun Jin Cho(Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea,)
• Pyung Gyun Shin( Division of Mushroom Research, IIHHS, RDA, Eumseong 369-873, Korea) | Pyung Gyun Shin
• Young Bok Yoo( Division of Mushroom Research, IIHHS, RDA, Eumseong 369-873, Korea) | Young Bok Yoo
• Young Un Cho( Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea) | Young Un Cho
• Soo Jeong Cho( Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea) | Soo Jeong Cho