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소에서 정자활성, 수정 양상 및 착상전 지속적 수정란 발달에 있어서 환삼덩굴 추출액의 효과
Effect of Humulus japonicus Extract on Sperm Motility, Fertilization Status and Subsequent Preimplantation Embryo Development in Cattle
- 언어KOR
- URLhttps://db.koreascholar.com/Article/Detail/279957
Humulus japonicus is an ornamental plant in the Cannabaceae family. Although the mode of action of Humulus japonicus is not fully understood, a strong relationship was observed between anti-inflammatory and anticancer in some types of cells. Recent studies also have shown that Humulus japonicus possesses anti-inflammatory activities and may significantly improve antioxidant potential in Raw 264.7 macrophage cells. Thus, the aim of this study was eva-luated the effect of Humulus japonicus extract on sperm motility and subsequent preimplantation developmental com-petence of the bovine embryos. After in vitro maturation, the oocytes with sperms were exposed in in vitro fertilization (IVF) medium supplemented with Humulus japonicus extract (0.01, 0.05, 0.1 μg/mL, respectively) for 1 day. In our results, exposure of IVF medium to Humulus japonicus extract did not affect sperm motility and percentage of pene-trated oocytes but ROS intensity was significantly decreased by 0.01 μg/mL compared with other groups (p< 0.05). Moreover, treatment with 0.01 μg/mL of Humulus japonicus extract was higher the frequency of blastocyst formation than the any other groups (p<0.05). Otherwise, treatment with 0.01 μg/mL of Humulus japonicus extract not increased the total cell number but reduced apoptotic-positive nuclei number. In conclusion, our results indicate that supple-mentation of Humulus japonicus extract in IVF medium may have important implications for improving early embryo-nic development in bovine embryos
서 론
재료 및 방법
배양액
정자의 운동성 평가
체외성숙, 수정 및 배양
ROS 발현 수준 분석
TUNEL 분석
실험 설계
통계처리
결 과
소 정자의 활동성 및 체외수정 양상 및 배발달에 있어서 환삼덩굴 추출액 처리 효과
체외수정 시 환삼덩굴 추출액 처리에 의해 생산된 수정란의 질적 분석
고 찰
인용문헌
