In this study, to further understand the mechanism of animal growth and to develop a miniature transgenic animal model, we constructed and tested tetracycline-inducible RNAi system using shRNA targeting the mRNA of mouse insulin-like growth factor (mIGF-1) or mouse growth hormone receptor (mGHR) gene. Quantitative real-time PCR analysis of mouse liver cell (Hepa1c1c7) cells transfected with these vectors showed 85% or 90% of expression inhi-bition effect of IGF-1 or GHR, respectively. In ELISA analysis, the protein level of IGF-1 in the cells expressing the shRNA targeting IGF-1 mRNA was reduced to 26% of non-transformed control cells. Unexpectedly, in case of using shRNA targeting GHR, the IGF-1 protein level was decreased to 75% of control cells. Further experiments are needed to explain the lower interference effect of GHR shRNA in IGF-1 protein. Accumulated knowledge of this approach could be applicable to a variety of related biological area including gene function study, gene therapy, development of miniature animals, etc.

ABSTRACT
 서 론
 실험 방법
  shRNA 합성
  생쥐 shRNA를 도입한 Tet Vector System의 유전자 발현의 Knock-Down 효과 검정
 결 과
  Tetracycline 유도적인 Retrovirus Vector System을 이용한 mIGF-1i의 Knock-Down 효과 검정
  Tetracycline 유도적인 Retrovirus Vector System을 이용한 mGHRi의 Knock-Down 효과 검정
  단백질 수준에서의 mIGF-1i와 mGHRi의 Knock-Down 효율 양상 확인
 고 찰
 인용문헌

• 손혜진(대구가톨릭대학교 의과대학 생리학교실) | Hye Jin Son
• 구본철(대구가톨릭대학교 의과대학 생리학교실) | Bon Chul Koo
• 권모선(대구가톨릭대학교 의과대학 생리학교실) | Mo Sun Kwon
• 이영만(대구가톨릭대학교 의과대학 생리학교실) | Young Man Lee
• 김태완(대구가톨릭대학교 의과대학 생리학교실) | Teoan Kim Corresponding author