To profile the proteome in porcine plasma, blood samples were collected from adult male barrows and those plasma were retrieved. For the depletion or pre-fractionation of high-abundance proteins, plasma samples were treated with commercial kits. Then, protein profiling was initiated using one and two-dimensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the results, more than forty six proteins were identified and the reference map was constructed. The pre-treatment for the removal of high-abundance proteins caused the changes in 2-DE images and some of the proteins were newly uncovered after the most of high abundant proteins were removed. However, it is expected for further steps necessary to identify more low-abundance proteins that may contain potential bio-markers.

• Jin Young Jeong(Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
• Jin Sun Nam(Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
• Mi Rim Park(Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
• Jang Mi Kim(Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
• Hak Jae Jeong(Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration)
• Kyung Woon Kim(Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration)
• Hyun-Jeong Lee(Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) Corresponding author