As a preceding study for investigating the influence of sound wave stimulus on Arabidopsis thaliana metabolomics, the polar secondary metabolomes of the plant were determined using high performance liquid chromatography coupled with tandem mass spectrometry. A total of 10 polar secondary metabolomes were characterized and quantified. Among them, 4 metabolomes, p-coumaroylagmatine isomer (7 and 8), p-coumaroylagmatine isomer (9 and 10) were identified in the plant for the first time. The validation was conducted in terms of linearity, recovery, precision, limit of detection (LOD) and limit of quantification (LOQ). The validated method was applied to the simultaneous quantification of the 10 polar secondary metabolomes.

• Young-Ah Cho(Dept. of Chemistry and Research Insti. of Life Sci., Gyeongsang National Univ.)
• Se-min Park(Dept. of Chemistry and Research Insti. of Life Sci., Gyeongsang National Univ.)
• Dong-Won Bae(Center for Research Facility, Gyeongsang National Univ.)
• On-Nuri Seo(Dept. of Chemistry and Research Insti. of Life Sci., Gyeongsang National Univ.)
• Ji-Eun Lee(Dept. of Chemistry and Research Insti. of Life Sci., Gyeongsang National Univ)
• Sung-Woo Jeon(Dept. of Chemistry and Research Insti. of Life Sci., Gyeongsang National Univ)
• Young-Sang Kwon(Division of Applied Life Science (BK21 program), Plant Melecular Biology & Biotechnology Research Center, Gyeongsang National Univ.)
• Jae-Yul Cha(Department of Applied Biology and Institute of Agricultural & Life Science, Gyeongsang National Univ.)
• Han-Hong Bae(School of Biotechnology, Yeungnam Univ.) Corresponding author
• Sung-Chul Shin(Dept. of Chemistry and Research Insti. of Life Sci., Gyeongsang National Univ.) Corresponding author