The potential application of surface-enhanced Raman spectroscopy (SERS) was investigated to detect Salmonella Typhimurium and Escherichia coli. The silver colloidal suspension with optimum optical characteristics was obtained for SERS detection. The SERS spectra were collected the wavelengths from 500 to 1200 cm-1. The characteristic SERS spectra were obtained from both S. Typhimurium and E. coli; however the observed SERS peak profiles for both pathogens exhibited similar peak profiles. Therefore, the results suggest that even though SERS possesses the potential capability to detect pathogens rapidly, further study is required to resolve the ambiguous spectra profiles for S. Typhimurium and E. coli.

ABSTRACT
 I. Introduction
 II. Materials and Methods
  2.1 Preparation of silver colloid
  2.2 Bacterial preparation
  2.3 Measurement of surface-enhanced Raman spectroscopy
 III. Results and Discussion
  3.1 UV-VIS spectrum of silver colloid
  3.2 Surface-enhanced Raman spectroscopic spectra
  3.3 Time-dependent spectral evolution of SERS
 » Literature cited

• Mi-Kyung Park(School of Food Science and Biotechnology, Kyungpook National University)
• Minseo Park(Department of Physics, Auburn University) Corresponding author