This research analyzed dynamic responses of the preflex railroad bridge. Vertical deflection and acceleration induced by operating train loads and test train loads were measured. Deflection of bridge by train traveling satisfies deflection limitation regulation (L/800) about the concrete bridge, but compare with UIC standard, vibration acceleration happened fairly greatly. Also test result show that acceleration receives greatly effect about the speed than deflection. It must discuss about vibration acceleration problems for speed elevation hereafter.