논문 상세보기
pp.65-71
산소자유라디칼의 하나인 hydrogen peroxide(H2O2)에 대한 세포독성과 이에 대한 페놀화합물의 일종인 gallic acid의 영향을 cell adhesion activity(CAA)를 비롯한 DPPH-radical scavenging activity, lactate dehydrogenase(LDH) activity에 의하여 분석하였다. 본 실험에서 H2O2는 배양 인체피부흑색종세포(SK-MEL-3)에 처리한 농도에 의존적으로 유의한 세포부착율의 감소를 나타냈으며 또한 고독성 효과를 보였다. 한편, H2O2의 산화적 손상에 대한 gallic acid의 방어효과를 조사하기 위하여 세포부착율과 LDH 활성을 조사하였다. 그 결과 gallic acid는 H2O2에 의하여 감소된 세포부착율을 유의하게 증가시킨 반면, LDH 활성은 유의한 감소를 보임으로서 항산화효과를 나타냈다. 더욱이 gallic acid는 DPPH-radical scavenging activity의 분석에서 유의한 자유라디칼 소거능을 보였다. 이상의 결과로 부터 H2O2는 배양 인체피부흑색종세포에 고독성을 나타냈으며 gallic acid와 같은 페놀화합물은 H2O2와 같은 산화적 손상을 방어하는데 효과적이었다.
To clarify the cytotoxicity of hydrogen peroxide(H2O2) on the cultured human skin melanoma cells(SK-MEL-3), cell adhesion effect was measured by colorimetric assay and also, the protective effect of phenol compound, gallic acid on SK-MEL-3 cells. For this study, SK-MEL-3 were grown in the media containning 3∼30uM concentrations of gallic acid for 12 hours, and cell adhesion activity(CAA) and lactate dehydrogenase(LD-H) activity was examined. And also, DPPH-radical scavenging activity was assessed on cultured SK-MEL-3 in these cultures. In this study, H2O2 significantly decreased cell adhesion activity dose-dependently. The midcytotoxicity value(MCV) was determined at 20uM of H2O2 following the treatment of K-MEL-3 with 10∼30uM of H2O2. In the protective effect of gallic acid on the cytotoxicity induced by H2O2, gallic acid increased cell adhesion activity which was decreased by H2O2-induced cytotoxicity, and also, LDH activity was decreased by gallic acid which was increased by H2O2-induced cytotoxicity. Gallic acid also showed DPPH-radical scaveng activity. From the above results, it is suggested that H2O2 had highly toxic effect on cultured SK-MEL-3 cells and gallic acid such as phenol compound showed the protection on the cytotoxicity induced by H2O2.
Ⅰ. 서론
Ⅱ. 재료 및 방법
Ⅲ. 결과 및 고찰
Ⅳ. 적요
Ⅴ. 인용문헌
