논문 상세보기
pp.131-137
활성산소 (reactive oxygen species, ROS)에 대한 현삼(Scrophulariae Radix, SR)추출물의 항산화 효과를 조사하기 위하여 NIH3T3 섬유모세포를 배양한 후 H2O2의 세포독성분석과 이에 대한 SR추출물의 영향을 항산화 측면에서 조사하였다. 본 실험에서 H2O2는 세포생존율의 감소함으로서 세포독성을 나타냈으며 이는 Borenfreund와 Puerner(1984)의 독성판정기준에 의하여 강독성인 것으로 나타났다. 한편, H2O2에 대한 SR추출물의 항산화 효과에 대한 분석에 있어서 SR추출물은 H2O2의 세포독성에 대하여 세포생존율의 증가를 비롯하여 LDH 활성감소 및 DPPH-자유기제거능을 나타냄으로서 항산화 효과를 나타냈다.
To evaluate the antioxidant effect of Scrophulariae Radix (SR) extract on reactive oxygen species (ROS)-induced cytotoxicity, XTT assay was done to examine the cell viability after NIH3T3 fibroblasts were grown with various concentrations of hydrogen peroxide (H2O2). And also, the antioxidant effects of SR on ROS were examined by cell viability, lactate dehydrogenase (LDH) activity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity in these cultures. In this study, ROS decreased cell viability in dose- and time-dependent manners in these cultures and the midcytotoxicity value (MCV) of H2O2 was determined at concentration of 50 uM after NIH3T3 fibroblasts were incubated with 50～90 uM H2O2 for 8 hours, respectively. H2O2 was highly toxic on cultured NIH3T3 fibroblasts by toxic criteria. In the antioxidant effect of SR extract, SR extract showed DPPH radical scavenging activity, the decreased LDH activity and increased remarkably cell viability compared with H2O2-treated group. From these results, it is suggested that H2O2-mediated cytoxicity was highly toxic and SR extract showed the protective effect on H2O2-induced cytotoxicity by antioxidant effects such as DPPH radical scavenging activity, the decreased LDH activity and the increased cell viability in these cultures.
Ⅰ. 서론
Ⅱ. 재료 및 방법
Ⅲ. 결과
Ⅳ. 고찰
Ⅴ. 적요
Ⅵ. 인용문헌
