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고려인삼 신품종 '천량' 특이적 DNA 판별 마커 개발 KCI 등재

A Rapid Identification of Korean Ginseng Cultivar, Cheonryang, using Specific DNA Markers

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한국약용작물학회지 (Korean Journal of Medical Crop Science)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
초록

This study describes the efficient method for the discrimination of 'Cheonryang' in Panax ginseng Meyer using a STS primer. A total of 208 STS primers were applied to polymerase chain reaction (PCR) amplification for discriminating Korean ginseng cultivars. Co-dominant polymorphic band patterns were generated with two primers, MFGp 0019, MFGp 0248, and successful identification of 'Cheonryang' was achieved from out of 11 Korean ginseng cultivars. Two different sizes of DNA band patterns were detected with MFGp 0019 primer. Ten Korean ginseng cultivars shared the same size of amplified DNAs (389 bp), but 'Cheonryang' showed a different size. Thus 'Cheonryang' can be efficiently distinguished from the other ten ginseng cultivars by using the MFGp 0019 primer. In the case of MFGp 0248, two different sizes of DNA band patterns were detected in the eleven ginseng cultivars. Same sized amplified DNA bands (307 bp) were shown in five cultivars (Chunpoong, Gopoong, Kumpoong, Cheongsun, Sunhyang) and 254 bp sized DNA bands were identified in the other 6 cultivars (Yunpoong, Sunpoong, Sunun, Sunone, Cheonryang, K-1). In conclusion, the two STS primers, MFGp 0019, and MFGp 0248, provide a rapid and reliable method for the specific identification of 'Cheonryang' cultivar from a large number of samples.

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ABSTRACT
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저자
  • 조익현(농촌진흥청 국립원예특작과학원 인삼특작부) | Jo, Ick Hyun
  • 김영창( 농촌진흥청 국립원예특작과학원 인삼특작부) | 김영창
  • 김장욱( 농촌진흥청 국립원예특작과학원 인삼특작부) | 김장욱
  • 이승호( 농촌진흥청 국립원예특작과학원 인삼특작부) | 이승호
  • 임지영( 농촌진흥청 국립원예특작과학원 인삼특작부) | 임지영
  • 문지영( 국립농산물품질관리원 시험연구소) | 문지영
  • 노봉수( 서울여자대학교 식품공학과) | 노봉수
  • 현동윤( 농촌진흥청 국립원예특작과학원 인삼특작부) | 현동윤
  • 김동휘( 농촌진흥청 국립원예특작과학원 인삼특작부) | 김동휘
  • 김기홍( 농촌진흥청 국립원예특작과학원 인삼특작부) | 김기홍
  • 방경환(농촌진흥청 국립원예특작과학원 인삼특작부) | Bang, Kyong Hwan Corresponding author