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Evaluation of Resistance to TYLCV (Tomato Yellow Leaf Curl Virus) in Tomato (Lycopersicum esculentum Mill.) Breeding Lines KCI 등재

토마토 육성계통의 TYLCV 저항성 평가

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한국국제농업개발학회지 (The Journal of the Korean Society of International Agriculture)
한국국제농업개발학회 (The Korean Society Of International Agriculture)
초록

토마토 TYLCV는 병이 발생되면 약제나 다른 방법으로 방제가 어려우므로 내병성 품종의 개발이 반드시 필요하다. 특히, TYLCV 저항성 유전자 Ty-1,2,3(부분우성)을 한 개체 내로 집적을 시켜서 저항성이 증대된 품종의 개발이 필요하다. 토마토 TYLCV 저항성 11-TY1-AV 등 7계통을 대상으로 보독인 담배가루이를 이용하여 표현형을 조사하였다. 이병성 대비(11-TY5-AV; 슈퍼썬로드)와 F3 세대 3계통 및 F2 세대 4계통을 TYLCTHV 균주를 이용하여 접종하였다. 접종 13일 후에 이병성 대비가 100% 이병증상을 나타내었으며, 이때 11-TY4-AV는 48개체 중에서 23개체가 이병되었으며, 11-TY6-AV는 42개체 중에서 14개체, 11-TY7-AV는 48개체 중에서 14개체, 11-TY8-AV는 48개체 중에서 15개체가 이병 되었다. F2 세대 4 집단 모두 p>0.05 수준에서 TYLCV 접종 후 표현형이 정상적인 유전분리비(3:1)를 보이는 것을 확인 하였다. 또, TYLCV에 저항성인 동일한 자원을 부계로 사용하더라도 모계로 어떤 것을 사용 하느냐에 따라서 저항성 정도가 다르게 나타나는 것을 확인할 수 있었다.

Tomato TYLCV is difficult to prevent by chemicals or other methods Thus, developmentof resistant cultivar is certainly needed. Especially, a resistant cultivar is required that show increased resistance through the accumulation of individual TYLCV resistance gene, Ty-1,2 and 3 (partial dominance). The phenotype of resistance was surveyed by the infection of whiteflies against 7 lines such as TYLCV resistant line (11-TY1-AV), susceptibility-control cultivar (11-TY5-AV), 3 lines of F3-generation and 4 lines of F2-generation. These cultivar and lines were artificially inoculated by TYLCTHV strain. The susceptible symptom could be confirmed at one week after the inoculation. After 10 days of inoculation, 83% of susceptible control showed the infected symptom. After 13 days, 100% of the control showed the TYLCV symptom. However, 16 individuals was infected out of 44 individuals in 11-TY4-AV and 12 individuals was infected out of 38 individuals in 11-TY6-AV. Eight individuals was infected out of 43 individuals in 1-TY7-AV, and 11 individuals was infected out of 42 individuals in 11-TY8-AV. Regarding these results, the genetic segregation ratio (3 : 1) in phenotype of 4 lines of F2 generation is following the Mendelism in all lines. Also, it could be confirmed that the resistance level is depending on maternal lines even with same paternal line that is resistant to TYLCV.

저자
  • Haksoon Choi(Vegetable Research Division, National Institute of Horticultural & Herbal Science) | 최학순 Corresponding author
  • Andreas Ebert(AVRDC - The World Vegetable Center, PO Box 42 Shanhua, Tainan 74199, Taiwan) | 안드레아스 이버트
  • Wen Shi Tsai(AVRDC - The World Vegetable Center, PO Box 42 Shanhua, Tainan 74199, Taiwan) | 웬시 차이
  • Eun Young Yang(Vegetable Research Division, National Institute of Horticultural & Herbal Science) | 양은영
  • Woo Moon Lee(Vegetable Research Division, National Institute of Horticultural & Herbal Science) | 이우문
  • Yun- Chan Huh(Vegetable Research Division, National Institute of Horticultural & Herbal Science) | 허윤찬
  • Hee Ju Lee(Vegetable Research Division, National Institute of Horticultural & Herbal Science) | 이희주
  • Dong Kum Park(Vegetable Research Division, National Institute of Horticultural & Herbal Science) | 박동금
  • Myeong Cheoul Cho(Vegetable Research Division, National Institute of Horticultural & Herbal Science) | 조명철
  • Bong Nam Jung(Apple Research Station, National Institute of Horticultural & Herbal Science) | 정봉남
  • Seung Ryong Cheong(Vegetable Research Division, National Institute of Horticultural & Herbal Science) | 정승룡