논문 상세보기

Genetic Distances and Variations of Three Clupeid Species Determined by PCR Technique KCI 등재

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/285834
서비스가 종료되어 열람이 제한될 수 있습니다.
Development & Reproduction (발생과 생식)
한국발생생물학회 (The Korea Society Of Developmental Biology)
초록

In this study, seven oligonucleotides primers were shown to generate the shared loci, specific loci, unique shared loci to each species and shared loci by the three species which could be obviously calculated. Euclidean genetic distances within- and between-species were also calculated by complete linkage method with the sustenance of the hierarchical dendrogram program Systat version 13. The genomic DNA isolated from herring (Clupea pallasii), Korean anchovy (Coilia nasus) and large-eyed herring (Harengula zunashi), respectively, in the Yellow Sea, were amplified several times by PCR reaction. The hierarchical dendrogram shows three chief branches: cluster 1 (PALLASII 01, 02, 03, 04, 06 and 07), cluster 2 (NASUS 08, 09, 10, 11, 12, 13 and 14), and cluster 3 (ZUNASHI 15, 16, 17, 18, 19, 20, 21 and PALLASII 05). In three clupeid species, the shortest genetic distance displaying significant molecular difference was between individual PALLASII no. 03 and PALLASII no. 02 (0.018). Individual no. 06 of PALLASII was most distantly related to NASUS no. 11 (genetic distance = 0.318). Individuals from herring (C. pallasii) species (0.920) exhibited higher bandsharing values than did individuals from Korean anchovy (C. nasus) species (0.872) (P<0.05). As a result, this PCR analysis generated on the genetic data displayed that the herring (C. pallasii) species was widely separated from Korean anchovy (C. nasus) species. Reversely, individuals of Korean anchovy (C. nasus) species were a little closely related to those of large-eyed herring (H. zunashi) species.

저자
  • Sang-Hoon Cho(Dept. of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
  • Jong-Man Yoon(Dept. of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) Corresponding Author