논문 상세보기

Effect of Nicotinic Acid on Fresh Semen Characteristics in Miniature Pigs KCI 등재

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/286393
  • DOIhttps://doi.org/10.12750/JET.2014.29.4.385
구독 기관 인증 시 무료 이용이 가능합니다. 4,000원
한국동물생명공학회지 (구 한국수정란이식학회지) (Journal of Animal Reproduciton and Biotechnology)
한국동물생명공학회(구 한국수정란이식학회) (Journal of Animal Reproduction & Biotechnology)
초록

Objective of this study was to investigate the effect of nicotinic acid (NA) on the characteristics in fresh semen of miniature pig. We evaluated viability, acrosome reaction and mitochondrial integrity of sperm on 0, 3, 7 and 10 days during storage period with nicotinic acid. As results, the survival rate of sperm in 15 mM NA (day 3, 87.8 ± 1.2%; day 5, 84.0 ± 2.7%; day 7, 82.2 ± 0.9%) and 30 mM NA (day 3, 87.7 ± 0.3%; day 5, 84.4 ± 2.5%; day 7, 82.3 ± 0.7%) groups were higher than control and 5 mM NA groups in 3, 7 and 10 days of semen storage. The NA-treated sperm on 10 day was used day for observing acrosome integrity. The survival sperm with acrosome reaction was higher in 30 mM NA group (day 3, 2.7 ± 0.2%; day 5, 3.3 ± 0.6%; day 7, 11.4 ± 0.3%) than in the control, significantly (P<0.05). Moreover, the live sperm with mitochondrial integrity was higher in whole treatment groups of NA than control group, significantly (P<0.05). Specially, most mitochondrial integrity on 10 day of semen storage was significantly higher in 30 mM NA group (90.2 ± 1.6%) than other treatment groups (control, 81.8 ± 3.1%; 5 mM NA, 83.4 ± 3.0%; 15 mM NA, 89.1 ± 0.7%, P<0.05). In conclusion, supplement of NA in liquid semen of miniature pig can improve and maintain semen quality, such as viability, acrosome reaction, and mitochondria integrity.

저자
  • Yeon-Ju Lee(College of Animal Life Science, Kangwon National University)
  • Sang-Hee Lee(College of Animal Life Science, Kangwon National University)
  • Eunsong Lee(College of Veterinary Medicine, Kangwon National University)
  • Seung Tae Lee(College of Animal Life Science, Kangwon National University)
  • Hee-Tae Cheong(College of Veterinary Medicine, Kangwon National University)
  • Boo-Keun Yang(College of Animal Life Science, Kangwon National University)
  • Seunghyung Lee(Institute of Animal Resources, Kangwon National University)
  • Choon-Keun Park(College of Animal Life Science, Kangwon National University) Corresponding author