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셀룰로오스 및 다시마의 효소 가수분해 특성 KCI 등재

Characteristics of Enzymatic Hydrolysis of Microcrystalline Cellulose and Laminaria japonica

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한국폐기물자원순환학회지 (Journal of Korea Society of Waste Management)
한국폐기물자원순환학회 (Korea Society Of Waste Management)
초록

In this study, microcrystalline cellulose, which is a cell wall polysaccharide commonly contained in sea algae (brown algae, red algae and green algae), is used in substitution for cellulose and is hydrolyzed with seven enzymes available in the market. The seven enzymes selected are Viscozyme® L, Celluclast® 1.5 L, Saczyme, Novozym® 33095, Fungamyl® 800 L, Driselase® Basidiomycetes sp., and Alginate Lyase. To maximize the production of the reducing sugar by hydrolysis with each enzyme, we optimized the quantity of enzymes, reaction time, pH, and reaction temperature as four independent variables, and the reducing sugar production rate as a dependent variable, utilizing response surface methodology (RSM) to optimize the enzyme hydrolysis reaction conditions. Among the tested enzymes, the production rate of reducing sugar by Celluclast® 1.5 L was the highest. Hence, the predicted optimum conditions (8.5 % enzyme, reaction time 27.6 h, pH 4.1 and reaction temperature 44.1oC) were directly applied to Laminaria japonica and proved the predicted optimum conditions with experiments. Under the optimum conditions, the sugar yield of 137.6 mg/g-Laminaria japonica (experimental value) was obtained.

저자
  • 박은영(서울시립대학교 대학원 에너지환경시스템공학과) | Eun-Young Park
  • 김용진(목포해양대학교 환경·생명공학과) | Yong-Jin Kim Corresponding author
  • 정승미(서울시립대학교 대학원 에너지환경시스템공학과) | Seung-Mi Jeong
  • 이동훈(서울시립대학교 대학원 에너지환경시스템공학과) | Dong-Hoon Lee