Forest waste was interested as biomass to produce new renewable energy among various materials. To find appropriate conditions of the bio-ethanol production, acid hydrolysis and glucose fermentation experiments were conducted under various conditions. The acid-hydrolysis experiment results show that yield of glucose were increased as raise of temperature, acid concentration and reaction time. As a result, the optimal conditions for producing glucose from forest waste was under 110oC, 35%, and 100 min, respectively. The yield of glucose, which was generated from acid-hydrolysis experiment, was 2.419 mg/g·g from softwood and was 1.192 mg/g·g from hardwood. Also, it was investigated that acetic acid was more efficient than sulfuric acid for acid-hydrolysis process.