Sacbrood disease is a viral disease on honey bee larvae Apis cerana. Diseased larvae fail to pupae and to be dead at old larvae and pre-pupae stage. Currently, there is no remedy to control sacbrood disease. In this study we conducted to observe sacbrood disease on Apis cerana colonies from June to September, 2014 at the A. cerana apiary of NAAS, and using biological measure to treat this disease. Our study results were showed that sacbrood disease infected A. cerana colonies in all months of observation. The percentage of infected colonies was from 33.3% up to 100%. Controlling sacbrood disease by requeen measure, the percentage of recovered colonies was 57.1 % while of this by cage queen measure was only 28.6 %.