Enhanced green fluoresce protein gene (egfp) was expressed in Beauveria bassiana ERL836 based on the Agrobacterium tumefaciens-mediated transformation (AtMT) method in this study. The ERL836 transformants were generated with pCambia-egfp binary vector. Ten transformants were randomly selected and analyzed for the T-DNA insertion and gene expression. The results revealed that 60% of the fungal putative transformants were inserted by the T-DNA fragment. Of these transformants, 33.33% (2 transformants) expressed the egfp gene. The egfp transformants showed strong green fluorescence with different expression levels. The results of this study could provide a reference for foreign protein expression in B. bassiana by using the AtMT method.

• Yu-Shin Nai(Department of Agricultural Biology, Chonbuk National University)
• Se Jin Lee(Department of Agricultural Biology, Chonbuk National University)
• Sihyeon Kim(Department of Agricultural Biology, Chonbuk National University)
• Ho-Jong Ju(Department of Agricultural Biology, Chonbuk National University)
• Yeon-HoJe(School of Agricultural Biotechnology, Seoul National University)
• Jae Su Kim(Department of Agricultural Biology, Chonbuk National University)