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Analysis of Transcriptome of Teratocyte in Cotesia plutellae

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/288642
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

Cotesia plutellae has been known as a natural enemy against the Diamondback moth, Plutella xylostella via laying eggs into a larva. When the larva hatches from the egg, teratocytes also are released and expected to work as immune suppressor via secreting immune suppressive factors. In order to analyze the gene expression in teratocytes, total RNAs were isolated and genes expressed in the teratocyte were sequenced by Illumina HiSeq2000 RNASeq analysis. The information on RNA sequences was assembled by Trinity and contigs were annotated by Blast analysis. The levels of gene expression were calculated by FPKM. Approximately, 6.3 Gbs were obtained and 34,686 contigs were found and annotated. Forty two percent of contigs were homologous to previously reported genes and classified by gene ontologies: the highly abundant components are metabolic process, biological regulation and cellular process in biological function; binding, catalytic activity and transporter activity in molecular function; cell part, membrane part and organelle in cellular function, respectively. In addition, some teratocyte transcripts of C. plutellae are related to host regulation such as immunosuppression and nutrition: Ankyrin repeat proteins, Serpin, protease, lipase, chitinase and scavenger receptor.

저자
  • Wook Hyun Cha(Department of Biology, KyungSung University)
  • Yonggyun Kim(Department of Agricultural Biology, Andong National University)
  • Dae-Weon Lee(Department of Biology, KyungSung University)