Beet armyworm, Spodoptera exigua are difficult to control using chemical insecticides because of the development of insecticide resistance. For eco-friendly beet armyworm managements, various control agents are required. Entomopathogenic fungus is one of promise control agents as an alternative to chemcal control agent. We conducted bioassays with entomopathogenic fungi to select high virulence isolate to larva of beet armyworm. The bioassay was used 150 entomopathogenic fungal isolates which were isolated from soil samples of nine provinces by insect-bait method using Galleria mellonella and Tenebrio molitor. . Three isolates, Metarhizium spp. FT83, FT89 and FT90 had high virulence as 84.6%, 100% and 100%, respectively, against S. exigua. The medial lethal time(LT50) of conidia of three isolates was 5.01, 2.99 and 2.92days respectively.