Among hemipteran insects which is the most important insect vector of plant viruses, small brown planthopper, Laodelphax striatellus, transmits the rice stripe virus (RSV) causing rice stripe disease. For effective control of RSV, it is important to understand interaction between RSV and L. striatellus. Therefore, in this study, expressed sequence tag (EST) databases were generated based on 454 GS-FLX pyrosequencing for comparative transcriptome analysis between nonviruliferous and RSV-viruliferous L. striatellus. By comparing the two EST libraries, we showed that 108 host genes were significantly up-regulated and 28 host genes were significantly down-regulated in viruliferous insects. Interestingly, genes encoding ribosomal proteins were mainly up-regulated in viruliferous L. striatellus, whereas genes related to translation were concentrated in the downregulated cohort. These RSV-dependently regulated genes may have important function in the behavior of planthopper or the transmission of RSV.

• Joo Hyun Lee(Department of Agricultural Biotechnology, Seoul National University)
• Jae Young Choi(Department of Agricultural Biotechnology, Seoul National University)
• Xue Ying Tao(Department of Agricultural Biotechnology, Seoul National University)
• Qin Liu(Department of Agricultural Biotechnology, Seoul National University)
• Song Eun Kim(Department of Agricultural Biotechnology, Seoul National University)
• Woo Jin Kim(Department of Agricultural Biotechnology, Seoul National University)
• Jae Su Kim(Department of Agricultural Biology, Chonbuk National University)
• Yeon Ho Je(Department of Agricultural Biotechnology, Seoul National University)