The cladistics analysis of subfamily Phylinae, based on molecular data analysis of 3655 base pairs of mitochondrial (16S, COI) and nuclear (18S, 28S) ribosomal DNA for 66 taxa in five tribes were conducted. Partitions of datasets were aligned using different algorithms implemented in MAFFT, further combined through sequence matrix and the combined dataset was analyzed using partitioned maximum likelihood. Clades in analytical method comprise six subfamilies of Miridae as outgroup: Isometopinae, Cylapinae, Bryocorinae, Orthotylinae, Deraeocorinae, and Mirinae, and five tribes of ingroup in subfamily Phylinae: Auricillocorini, Hallodapini, Phylini, Leucophoropterini, and Pilophorini. In analysis, tribe Auricillocorini as sister group to all remaining phylines. Most of the tribes are monophyly, except largest tribe phylini, which is clearly divided as paraphyly.