A growing interest in ecotoxicological study is on the development of tools and technical resources to assess or determine the effects of a variety of stresses on ecosystems. As many chemicals are synthesized and used for the various purposes, it is inevitable circumstance for organisms to meet the stressors in the environment. Thus, it is important for us to understand the impacts of the stressors to organisms and is essential to equip with a fast detecting system to predict of their behaviors. The high throughput technology using proteomic and metabolomic techniques has been introduced to satisfy such requirements to study the potential adverse effects of some chemicals. Ecotoxicproteomics and Ecotoxicometabolomics are discussed in this talk in terms of their possible role in ecotoxicological studies.