DNA barcode projects in Hexapoda have been initialized and progressed accumulating large number of mitochondrial gene sequences. However, due to large number of data, overview of DNA barcode projects was not conducted until now. Here we reported the current status of DNA barcode projects with the aid of Insect Mitochondrial Genome Database (IMGD; http://www.imgd.org/) which archives 128,562 partial mitochondrial gene sequences (PMEs) of Hexapoda. Among 37 mitochondrial genes, COI has been used popularly (22,379 PMEs;17.40 %) through all 33 orders. Through 513 researches, different parts of COI PME have been utilized differently along with hexapod orders. Inaddition, by calculating genetic divergences of COI PMEs, intra-species and inter-species in 21 hexapod orders were distinguished by 5% divergence and some of mitochondrial genes in certain order present higher genetic divergences than that of COI. Based on these results, we confirmed that DNA barcode is a use ful tool to identify hexapod species and several mitochondrial genes can be good molecular markers to support COI.