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First detection of deformed wing virus(DWV) from bumblebee (Bombus ignitus)

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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

We report for the first time the occurrence of DWV-infected bumble bees (Bombus ignitus). For the present study, the detection of DWV virus from the female and male bumble bee was investigated in the same colony. The Deformed wing virus (DWV) of honeybee (Apis mellifera) is closely associated with characteristic wing deformities, abdominal bloating, paralysis, and rapid mortality of emerging adult bees. Using specific RT-PCR protocols for the detection of DWV followed by sequencing of the PCR products we could demonstrate that the bumble bees were indeed infected with DWV. The virus was detected from Bombus ignitus, and its partial DWV gene was cloned and sequenced. The partial DWV gene encoding the polyprotein is 711-nt of 235 amino acid residues. The deduced nucleotide sequence of the polyprotein partial gene of DWV showed 96.9%, 96.2%, 96.8%, and 96.5% homology to other structure polyprotein partial gene of DWV from insects, respectively. Phylogenetic analysis further conformed that the deduced nucleotide sequence of the polyprotein partial gene of DWV divided to the outside tree. We describe the first time that presence of Deformed wing virus(DWV) from bumble bee(Bombus terrestris) in korea using RT-PCR.

저자
  • Yong Soo Choi(National Academy of Agricultural Science and Technology, R.D.A.)
  • Man Young Lee(National Academy of Agricultural Science and Technology, R.D.A.)
  • Hye Kyung Kim(National Academy of Agricultural Science and Technology, R.D.A.)
  • Nam-Suk Kim(National Academy of Agricultural Science and Technology, R.D.A.)
  • In-Pyo Hong(National Academy of Agricultural Science and Technology, R.D.A.)
  • Kyu-Ho Byeon(National Academy of Agricultural Science and Technology, R.D.A.)
  • Kwang Gill Lee(National Academy of Agricultural Science and Technology, R.D.A.)
  • Hyung Joo Yoon(National Academy of Agricultural Science and Technology, R.D.A.)