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Semi-Artificial Diet for Murgot Looper Moth, Ascotis selenaria, and their Developmental Characteristics
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- URLhttps://db.koreascholar.com/Article/Detail/291601
Murgot Looper Moth, Ascotis selenaria, is one of the worldwide pests in citrus plants. They usually damages citrus leaves and often makes young fruits injured. In Jeju, it has been discovered that A. selenaria is a pest of two major geometridae moths being occurred in citrus plant and they also had periodically occurred and damaged bean, asparagus, etc. However, physiological and ecological studies were rarely conducted on this species, which maybe resulted from the limitation of rearing method. Therefore, this study aimed on selecting the diet sources and developing mass-rearing method for A. selenaria, in order to make ease or spur a investigation related with A. selenaria. The major sources, selected among the series of semi-artificial diet, were corn and bean powder and adding yeast makes the larva developed well. Cholesterol, one of the minor sources, enhanced the development rate and lowered the mortality during 1 to 3 larva stage. Consequently the semi-artificial diet, suitable for rearing A. selenaria larva, was composed of 25g agar, 100g corn powder, 100g soybean powder, 30g yeast, 0.5g cholesterol, 2g Vanderzant's vitamin mixture, 2g Wesson's salt mixture, 2g sorbic acid, 2.5g methyl-4-hydroxybenzoate(MPH), 2g Ascorbic acid, and 1000 ml distilled water. A. selenaria larvae has 6 developmental stage and the width of head piece was 0.31 mm (1st instar) , 0.51 mm (2nd), 0.85 mm(3rd), 1.37 mm (4th), 2.14 mm (5th), and 3.19 mm (6th). The development period of A. selenaria, reared at 25℃ and 16L:8D photoperiod, was 4.7 day (egg), 3.58 day(1st instar), 1.88 day (2nd), 1.80 day (3rd), 2.46 day (4th ), 2.59 day (5th), 8.10 day (6th), 11.26 day (pupae) when citrus leaves were supplied to the larva. The larvae, fed with the artificial diet(mentioned above), showed similar developmental periods at each developmental stage as much as the case reared with citrus leaves, although the mortality was sometimes a little bit higher. The weight of pupae were 0.614g (artificial diet) and 0.427g (citrus stuff) and female adult moths lay 1094 eggs (artificial diet) and 1054 eggs (citrus stuff).
