Mechanical stimulation has been reported to improve cell growth in bone and cartilage regeneration. Various research groups have described their own bioreactors that stimulate cell-seeded scaffolds. We have developed a bioreactor capable of applying controlled compressive loading to a cell-encapsulated agarose hydrogel. To compare the effect of mechanical stimulation on agarose hydrogel, we used one control group (static mode) and five experimental groups. The results show that the intermittent_3sec conditions improve proliferation of cells better than the other conditions studied.

Abstract
 1. Introduction
 2. Fabrication of Bioreactor
 3. Materials and Methods
  3.1 Cell culture
  3.2 Fabrication of hydrogel scaffold
  3.3 Modes of compression stimulation
  3.4 Analysis of flow proliferation and fluorescence images
 4. Results and Discussions
 5. Conclusion
 Acknowledgment
 References

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• 이인환(충북대학교 기계공학부) | In Hwan Lee