Seismic isolation offers an attractive approach for reducing seismic loads in the building and in its components. This paper deals with the seismic isolation effects due to variation in period ratio between superstructure and isolation layer of the building. Seismic isolation effects of the moment-resisting frames with the various period ratio were studied by nonlinear time history analysis. It is recommended that the isolation period should be greater than 2.0 times of that of the superstructure for the effective seismic isolation.