E6 and E7 as two major oncoproteins of HPV can act together to produce several tumors, providing an evidence for the role HPV in oral squamous cell tumorogenesis. It is worthy to detect E6/E7 mRNA expression of HPV in oral carcinoma. The purpose of this study were to detect HPV mRNA in HNSCC cell lines, and to use these results to confirm oral squamous cell carcinoma. Semi-quantitative RT-PCR method for E7 mRNA expression in HNSCC cell lines should play an important role in detecting the early stage of oral squamous cell carcinoma.

I. INTRODUCTION
 II. MATERIALS and METHODS
  A. Culture Conditions
  B. Total RNA extraction from NHOK, IHOK,Caski cell line and HNSCC cell lines for RT-PCR
  C. RT-PCR reaction for cDNA
 III. RESULTS
 IV. DISCUSSION
 REFERENCES

• 이종헌(단국대학교 치과대학 구강병리학 교실) | Chong Heon Lee correspondence