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구강 편평세포암종에서의 p16INK4A와 p15INK4B 발현과 메틸화의 관계 KCI 등재

Expression of p16INK4A and p15INK4B in oral squamous cell carcinomas: relationship with methylation status

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  • URLhttps://db.koreascholar.com/Article/Detail/292801
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대한구강악안면병리학회지 (The Korean Journal of Oral and Maxillofacial Pathology)
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

p16INK4A and p15INK4B tumor suppressor genes are frequently altered in various human tumors. Hypermethylation of the promoter region of p16INK4A and p15INK4B seem to be the major mechanism of inactivation. To determine whether the change in p16INK4A and p15INK4B methylation status occur in oral squamous cell carcinomas (OSCCs) and benign oral epithelial hyperplasias, we analyzed 46 OSCCs and 20 benign oral epithelial hyperplasias by methylation-specific PCR. We also analyzed a subset of the samples for p16INK4A and p15INK4B protein expression by immunohistochemistry. The promoter region of p15INK4B was hypermethylated in 13 specimens of the 15 finally analyzed OSCCs and three specimens of the five analyzed benign oral epithelial hyperplasias. By immunohistochemical analysis, we confirmed the loss of p15INK4B expression of all hypermethylated specimens. The promoter region of p16INK4A was amplified by both an unmethylated- and a methylated-specific primers in just one OSCCs. The remaining specimens including 11 OSCCs and four benign oral epithelial hyperplasias were normally methylated. By immunohistochemistry, we analyzed the loss of p16INK4A expression in seven specimens of the 12 OSCCs and two specimens of the four benign oral epithelial hyperplasias. Except for one OSCC, however, all specimens showing loss of expression were normally methylated. These results suggest that loss of p16INK4A and p15INK4B protein expression play an important role in the development of both OSCCs and benign oral epithelial hyperplasias.

목차
I. 서 론
 II. 재료 및 방법
 III. 결 과
  1. methylation-specific PCR(MSP) 결과
  2. 면역조직화학적 염색결과
 IV. 고 찰
 V. 결 론
 참 고 문 헌
저자
  • 최철영(전북대학교 치과대학 구강병리학교실 및 구강생체과학연구소) | Cheol Young Choi
  • 추정엽(전북대학교 치과대학 구강병리학교실 및 구강생체과학연구소) | Jung Youb Chu
  • 류숙경(전북대학교 치과대학 구강병리학교실 및 구강생체과학연구소) | Suk Kyung Ryou
  • 조남표(전북대학교 치과대학 구강병리학교실 및 구강생체과학연구소) | Nam Pyo Cho 통신저자