Intracellular pathogens must maintain redox homeostasis against the antimicrobial actions of reactive oxygen and nitrogen species produced by host cells. This study proves that glutathione is required to promote survival of an enteric pathogen Salmonella under the conditions producing reactive oxygen or nitrogen species. Glutathione is the non-protein thiol compound distributed in a variety of organisms and possesses strong electron-donating capability to reduce intracellular redox environment. To examine the role of glutathione on Salmonella redox homeostasis under oxidative and nitrosative stress conditions, gshB gene encoding glutathione synthetase was mutated by the one-step PCR inactivation method. The growth of gshB mutant Salmonella producing virtually no glutathione was greatly impaired in the culture media containing either hydrogen peroxide or nitric oxide donors. The results suggest that physiological levels of glutathione can provide a fundamental capability to maintain redox homeostasis for Salmonella in surviving oxidizing conditions of host cells.

I. 서론
 II. 연구재료 및 방법
  1. 사용배지 및 화학재료
  2. 돌연변이체 제조
  3. 활성산소종/활성질소종에 대한 살모넬라의민감도 측정
 III. 결과 및 고찰
 IV. 참고문헌

• 방일수(조선대학교 치과대학 미생물학 및 면역학 교실) | Iel Soo correspondence