논문 상세보기
pp.61-66
The purpose of the present study is to investigate the optimal wavelength, frequency and energy density for set up the photobiologic treatment of periodontal disease. To establish the present study, λ scan of 500㎚ to 900㎚ was used to search the optimal wavelength for maximal proliferation of human gingival fibroblasts. Cell proliferation assay was carried out as MTT assay. Light intensity of 0.8 to 3.25mW, frequency of 0 to 584㎐ and 0 to 2hours was applied for investigation of optimal energy density, frequency and applied duration. Finally, 628㎚ with 1mW/cm2 for 1hour of LED irradiation resulted in maximal proliferation of gingival fibroblasts. These results suggest that LED irradiation on gingival fibroblast show different proliferation according to the condition of irradiation, and demonstrate that LED irradiation can control the quantity of cell proliferation.
II. 재료 및 방법
1. 치은섬유아세포의 배양
2. 치은섬유아세포의 흡수 스펙트럼 분석
3. 최적 광조사 조건의 탐색
4. 치은섬유아세포의 성장 분석
III. 결 과
1. 치은섬유아세포의 흡수 스펙트럼 분석
2. 파장의 변화에 따른 성장
3. 광 조사 세기의 변화에 따른 성장
4. 광 조사 시간의 변화에 따른 성장
5. 광 조사 주기의 변화에 따른 성장
IV. 고 찰
V. 결 론
VI. 참고문헌
