The organotypic raft culture model has been shown to be a useful method for examining the effects of biochemical manipulation on various epithelial cell types, using in vitro conditions that simulate the in vivo environment of the tissue of origin. To investigate this method as a model for photodynamic therapy (PDT), we cultures the oral squamous carcinoma cell line YD-10B on fibroblast-containing collagen gels at the air-liquid interface and assessed the efficacy of PDT using a photosensitiser 5-ALA-hexenyl ester. PDT effect on YD-10B organotypic raft cultures after twenty- four hours was determined. The number of residual cells was significantly reduced in comparison to control at all four different conditions. PCNA immunostaining and TUNEL assay revealed that PDT inhibited cell proliferation and increased apoptosis. These findings suggest that the organotypic raft culture model provides an effective and rapid laboratory method of investigating strategies for PDT on oral precancerous lesions and squamous cell carcinomas

I. 서론
 II. 연구재료 및 방법
  1. 구강암 세포의 삼차원 배양
  2. 삼차원 배양 구강암의 광역학 치료
  3. 병리조직, 면역조직화학 및 TUNEL 분석
  4. 통계 분석
 III. 연구결과
  1. 삼차원 배양(oraganotypic raft culture) 구강암조직
  2. 삼차원 배양 구강암의 광역학 치료 후 병리학적 소견
  3. 삼차원 배양 구강암의 광역학 치료후 PCNA 발현의면역조직화학적 평가
  4. 삼차원 배양 구강암의 광역학 치료후 TUNELassay 평가
 IV. 고찰
 V. 참고문헌

• 백종필(조선대학교 치과대학 병리학교실) | Jong Pil Baek
• 안상건(조선대학교 치과대학 병리학교실) | Sang Gun Ahn correspondence
• 윤정훈(조선대학교 치과대학 병리학교실) | Jung Hoon Yoon correspondence