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타액선 종양세포주에서 제2형 트랜스글루타민아제와 Nuclear Factor Kappa B의 연관성 KCI 등재

Transgluraminase 2 Expression Was Associated with Nuclear Factor Kappa in Salivary Gland Tumor Cell Line

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  • URLhttps://db.koreascholar.com/Article/Detail/293132
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대한구강악안면병리학회지 (The Korean Journal of Oral and Maxillofacial Pathology)
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

The transcription factor nuclear factor-kB (NF-kB) plays an important role in regulating cell growth, apoptosis, and metastatic functions. Constitutive activation of NF-KB has been observed in various cancers; however, molecular mechanisms resulting in such activation remain elusive. Numerous evidences showed that over expression of TGase 2 might be linked with constitutive activation of NF-KB. To understand the pathways responsible for constitutive activation of NF-kB is important for rational design of NF-kB inhibitors for cancer therapy. Human salivary gland adenocarcinoma has been most aggressive solid tumors. Current therapy does not significantly improve survival rates. Thus, to investigate new therapeutic modalities against this adenocarcinoma is necessary. The purpose of this study was to study a constitutive activation of NF-kB with the expression of TG2 in SGT cell line origianted from human salivary gland adenocarcinoma by TGase 2 activity, RT-PCR and immunoslot blotting method. SGT cell line showed the highest TGase 2 activity and NF-kB activation than any other cell line. All the cell lines showed increased NF-kB mRNA activation after A231027 treatment than that of control. In immunoslot blotting, SGT cell line showed NF-kB activation correlated with TGase 2 expression after A231027 and BSA. It suggested that there might be a direct correlation between TGase 2 expression and NF-kB activation in SGT cell line.

목차
I. INTRODUCTION
 II. MATERIALS and METHODS
  1. Cell Culture Condition
  2. Sample Preparation for TGase assay
  3. Conditions for TGase Assay
  4. Total RNA Extraction and RT-PCR Analysis
  5. RT-PCR reaction for cDNA
  6. Immunoslot Blotting
 III. RESULTS
 IV. DISCUSSIONS
 V. REFERENCES
저자
  • 김인수(Department of Oral Pathology, The Oral Aging Research Center, Dental College, Dankook University) | In Soo Kim
  • 박경주(Department of Oral Pathology, The Oral Aging Research Center, Dental College, Dankook University) | Gyeong Ju Park
  • 이종헌(Department of Oral Pathology, The Oral Aging Research Center, Dental College, Dankook University) | Chong Heon Lee correspondence