The incidence of multidrug-resistant strains of Staphylococcus aureus continues to increase in hospitals and the community all over the world. As the importance of miner surgery in dentistry gets greater recently, the frequency in use of antibiotics has been increasing. Although several cases of acquired infection in oral cavity in dental hospital has been reported, the research on isolation of S. aureus and evaluation of its antibiotic resistance from outpatients in the dental clinic has not been found yet. The aim of this study was to evaluate the antimicrobial resistance of S. aureus isolates. The isolation rate of S. aureus were 41.7% in dental hospital personnel, 60.4% in outpatients and 62% in dental hygiene students. S. aureus which showed resistance to penicillin were 88.6%, ampicillin 88.6%, erythromycin 8.6%, tetracycline 4.3%, oxacillin 4.3%, cefoteten 4.3%. Six strains of S. aureus showed the susceptible to all antibiotics tested. Sixty-four(91.4%) strains were multiple antibiotic resistance. Fifty-two strains(72.8%) resisted to AM-P. and Seven strains(10.0%) resisted to AM-P-E, AM-P-GM, AM-P-TE.