Glutamate-induced oxidative injury contributes to neuronal degeneration in many central nervous system (CNS) diseases, such as Parkinson’s disease, Alzheimer’s disease, epilepsy and ischemia. Inducible heme oxygenase (HO)-1 acts against oxidants that are thought to play a role in the pathogenesis of these diseases. The EtOH extracts of Viola mandshurica (NNMBS274), Viola patrinii (NNMBS275) and Viola papilionacea Pursh (NNMBS276), origin plants of Violae Herba, showed the potent neuroprotective effects on glutamate-induced neurotoxicity. Among them, NNMBS275, the extract of V. patrinii possessed the protective effects against glutamate toxicity by inducing the expression of heme oxygenase (HO)-1 in the mouse hippocampal HT22 cells. These results suggest that extracts of V. patrinii could be the effective candidates for the treatment of ROS-related neurological diseases. Furthermore, it is suggested that the protective effects of V. patrinii extract due to inducing the expression of HO-1 as an antioxidant/cytoprotective target

Ⅰ. 서론
 Ⅱ. 재료 및 방법
  1. 실험재료
  2. 시약 및 기기
  3. 시료의 제조
  4. HT22 세포배양 및 뇌 세포 보호활성 측정
  5. Western Blot Analysis
 Ⅲ. 결과
  1. 글루타메이트로 유발한 세포독성에 대한 보호 효과
  2. Heme oxygenase-1 단백질 발현 효과
  3. Heme oxygenase-1 발현을 통한 세포 보호 효과
 Ⅳ. 고찰
 Ⅴ. 결론
 Ⅵ. 참고문헌

• 이동성(원광대학교 약학대학) | Dong Sung Lee
• 리빈(원광대학교 약학대학) | Bin Li
• 이윤정(원광대학교 의약자원연구소) | Yun Jung Lee
• 천현자(원광대학교 의약자원연구소) | Hyun Ja Chun
• 류일환(원광대학교 의약자원연구소) | Il Hwan Ryu
• 지연주(원광대학교 의약자원연구소) | Yeon Ju Jee
• 채권우(원광대학교 의약자원연구소) | Gwon U Chae
• 김영숙(원광대학교 의약자원연구소) | Young Sook Kim
• 손지우(원광대학교 의약자원연구소) | Ji Ue Shon
• 강현규(원광대학교 의약자원연구소) | Hyun Gyu Kang
• 이성희(원광대학교 의약자원연구소) | Sung Hee Lee
• 안인파(원광대학교 의약자원연구소) | Ren Bo An
• 이호섭(원광대학교 의약자원연구소) | Ho Sub Lee
• 김윤철(원광대학교 약학대학, 원광대학교 의약자원연구소) | Youn Chul Kim correspondence